Training

Training In Various Areas Of Human Assisted Reproduction Techniques

Note:

Hands on Training in Andrology Lab Protocols

During this course program each participant receives one-on-one training to develop knowledge and skills in following areas:

• Semen sample collection and delivery.
• Semen analysis.
• Manual and Computer assisted semen analysis (CASA).
• Sperm morphology assessment (WHO and Tygerberg strict criteria).
• Fructose detection in seminal plasma (qualitative)
• Viability testing (Eosin & Nigrosin stain).
• 24 hrs survival test
• Hypo-osmotic swelling test.
• Leukocyte detection (Endtz Test/Peroxidase staining).
• Antisperm antibodies detection (Immunofluorescence test)*
• Media preparation
• Consent forms & documentation

Sperm preparation protocols:

• Preparation of ejaculated sperm for ART:
  - Removal of Seminal plasma
  - Double density gradient technique
  - Swim-up method
• Concentration method (for low count samples)
• Method of retrieval of sperm from retrograde ejaculate*
• Sperm retrieval and preparation of testicular biopsy specimens (rat or mouse specimens will be used)*

Cryopreservation protocols:

• Human spermatozoa freezing:
  - By vapour freezing method
  - By Planar technique
• Isolation and cryopreservation of sperm from testicular biopsy specimens*
• Safety guidelines for andrology laboratory.

* Demonstration

Mode of teaching:

• Lectures and multimedia presentations.
• Interactive discussions.

Each participant will receive:

• Course manual.
• Certificate of training

Duration of hands on training: 7-days

Hands on training in Assisted reproduction techniques (IVF/ICSI)

This course provides comprehensive hands on training in the laboratory practices of IVF and ICSI. The syllabus covers the following areas:

Hands-on Laboratory Training:

Culture techniques

• Preparation of culture dishes for IVF/ IVF-ICSI cases
• Embryo culture
• Blastocyst culture
• Preparation of media for Hyaluronidase
• Preparation of media for vitrification of embryos and blastocysts

IVF Techniques

• Pipette pulling and Pipetting techniques
• Ovum pickup from follicular fluid
• Oocyte handling
• Denudation of oocytes for ICSI
• Morphological assessment of oocytes
• IVF procedure
• ICSI: Intracytoplasmic sperm injection
• Assessment of oocyte fertilization
• Embryo handling
• Embryo grading
• Embryo transfer (loading of embryos into embryo transfer catheter)

Advanced IVF techniques:

• Assisted hatching (laser)*
• Embryo biopsy *
• Oocyte Spindle imaging*

Andrology Techniques

• Semen Analysis (manual/CASA)
• Sperm preparation for IUI/IVF/ICSI
• Method of retrieval of sperm from retrograde ejaculate *
• Testicular sperm retrieval (mouse)
• Consent forms & documentation

Duration of hands on training: 14-days

Mode of teaching:

• Lectures
• Multimedia presentations
• Interactive discussions
  

Each participant will receive:

• Certificate of training
  *Demonstration, mouse oocytes/sperm will be used.

Hands on training in cryopreservation techniques

During this course program each participant receives hands on training in the following cryopreservation techniques:
Human spermatozoa freezing:

• By vapour freezing method
• By Planar technique
• Isolation and cryopreservation of sperm from testicular biopsy specimens*

Vitrification:

• Embryos
• Blastocysts
• Preparation of vitrification media
• Consent forms & documentation

* Demonstration
Mode of teaching:

• Multimedia presentation
• Interactive discussions

Each participant will receive:

• Course manual
• Certificate of training
  

Duration of hands on training: 4-days