Facilities Provided

Stereomicroscope
Luig-newmann micromanipulator with Carlzeiss axiovert 200 M
Microscope
Narishige micromanipulator with Olympus Inverted Microscope Polscope – Spindle View
Florescent microscope Axiovert 200 with Co2 chamber for Time-lapse studies
Florescent microscope (Axioskope 2 motplus) for FISH analysis
Nuaire C02 incubators (3)
Fertilase laser for assisted hatching
Nuaire laminar flow cabinet
-300C freezers (2)
Systat 12 statistical software

Services

In vitro fertilization (IVF)
Intracytoplasmic sperm injection (ICSI)
GIFT
ZIFT
Assisted hatching
Pre-implantation genetic diagnosis (PGD)
Oocyte spindle imaging
Embryo vitrification
Blastocyst vitrification
Oocyte vitrification

Research Projects

1)Studies on survival and embryological competence of oocytes preserved using in-house improved oocyte vitrification technique.
2) Studies on Perinatal outcome of pregnancies following vitrified day 3 embryo transfers
3) Studies on evaluation of in-house improved blastocyst vitrification media.
4) Studies on multivariate analysis of human oocytes using polscope imaging system and its influence on embryonic development.
5) Studies on evaluation of in-house improved Embryo vitrification media.
6) Studies on Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles
7) Imaging studies of oocytes, zygotes, embryos and blastocysts.

Publications

1)Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles. J Assist Reprod Genet 2009; 26: 523–529.
2)Neonatal outcome after vitrified day 3 embryo transfers: a preliminary study. Fertil Steril 2009; 92 : 143–148.
3)Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: A preliminary study using PolScope imaging. Reproductive BioMedicine Online 2007; 14: 166-174.
4) Successful pregnancy with laparoscopic oocyte retrieval and in-vitro fertilisation in mullerian agenesis. Singapore Med J 2006; 47: 329-331. 5Vitrification: An Emerging Technique for Cryopreservation in Assisted Reproduction Programmes. Embryo Talk 2006; 1: 210-227.
6Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates. Reproductive BioMedicine Online 2005; 11: 434–437.
7Vitrification of human embryos-Is it safe for clinical use? In Frozen Life: A Comprehensive Manual of Cryobiology for Assisted Reproduction and Stem Cells Edited by Pankaj Talwar, JP Medical Publishers, pp: 369-372, 2009.
8Vitrification of embryos In Atlas of Human Assisted reproductive Technologies edited by mangala telang, JP Medical Publishers, pp: 195-202, 2007.

Team

Dr. Madan

Dr. Jayprakash

Dr. Murali Krishna