Facilities Provided

Sperm class analyzer (CASA)-Microptics
Nikon phase contrast microscope (E-200)
Nikon phase contrast microscope (YS-100)
Lawrence & Mayo brightfield microscope (Lynx)
Labomed brightfield microscope
Planer for sperm cryopreservation
Arpeege storage cryocontainers (10 nos)
Hot airoven (02 nos)
IBP liq N2 containers (04 nos)

Sample Collection

Instructions for semen sample collection and delivery to Andrology LAB

1. The couple should abstain from any sexual activity for 2 to 5 days prior to collection of the semen sample. Ideally, it should be more than 2 days from a previous ejaculation and not more than 7 to 10 days.

2. Please collect the semen sample container from reception counter of the KIVF.

3. Patient is advised to use container provided by the clinic authorities and not to use any other container for semen sample collection

4. The sample should be collected by masturbation directly into a sterile, clean and dry container. If bringing the sample from home, collect the container from reception counter and after semen collection place specimen container upright in a plastic bag, with the lid securely tightened, and keep specimen close to body temperature by transporting close to the body. The specimen should not be placed in a purse, pocket, or briefcase. Bring the specimen to the laboratory within one hour of collection. Avoid exposing the specimen to extreme temperatures.

5. A semen sample should be collected only after you have washed your hands and penis with soap and water, being sure to rinse away all of the soap residue. Dry thoroughly before collecting the sample

6. Lubricants should not be used to aid in the collection of the sperm as they may be toxic to sperm.

7. Condoms should not be used for semen collection because they contain agents that kill sperm.

8. Interrupted intercourse should not be performed for specimen collection as this may results in the loss of the most critical portion (first portion) of the ejaculate and the specimen may be contaminated with cells or bacteria from the vagina. If you need to collect a sample with intercourse, you can purchase non-toxic condoms and use these for sample collection. If you collect a sample in this type of condom, drop the whole condom into one of our sterile containers and bring this into the laboratory.

9. If a pubic hair or thread of clothing accidentally falls into the container, do not attempt to remove it. The lab will remove it using sterile techniques.

10. If a portion of the sample was lost during collection, DO NOT attempt to transfer it to the container. Inform the lab personnel about the spill.

Research Projects

1) Studies on effect of Non insulin dependent diabetes mellitus on sperm morphological and functional characteristics, nuclear DNA integrity and outcome of assisted reproductive technique.
2) Studies on evaluation of in-house improved Tris-eggyolk buffer media on cryosurvival of spermatozoa
3) Studies on evaluation of in-house improved sperm chromatin dispersion technique
4) A comparative study of sperm DNA fragmentation in oligospermic, astheneospermic and teratospermic semen samples using SCD technique
5) Studies on evaluation of in-house improved Embryo vitrification media.
6) Studies on Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles
7) Imaging studies of oocytes, zygotes, embryos and blastocysts.

Publications

1) Noninsulin-dependent diabetes mellitus: effects on sperm morphological and functional characteristics, nuclear DNA integrity and outcome of assisted reproductive technique. Andrologia. 2011 Aug 2. doi: 10.1111/j.1439-0272.2011.01213.x. [Epub ahead of print]

Team

Mrs. Mythili
Mr. Ramana
Mr. Ramesh
Mrs. Renuka